Immunologic identification and reconizing of allergens from the shrimp Litopenaeus Vannamei (Boone, 1931) (Crustacea; Decapoda)

by Magalhães Madeira, Ana Verena

Sea-foods, especially crustaceans, are frequently reported as inductors of allergic reactions in food- sensible peoples. A despite of the frequency and clinic importance, the allergens responsible for these reactions are quite know. Allergens identification and characterization studies reveal tropomiosins as the principal allergens of shrimp, with molecular weight between 34 and 38kD. In other hand, studies about immunologic recognizing of these allergens show cross reaction between allergens of various crustaceans and even others sea-foods. This work used fifty human sera from a particular clinical analyses laboratory in Salvador, to do the identification of allergens from the shrimp L. vannamei, through Immunoblotting, and to characterize the quantitative recognizing of these allergens by sera, through ELISA. Frequency of positive results to the allergens FX2 e F24 in commercials kits was determinate in this sera bank. The major allergen of the cultivate shrimp Litopenaeus vannamei was identificated as a 44kD protein, recognized by 100% of patients with clinical diagnostic of shrimp allergy. At different frequencies, 17, 21, 24, 27, 29, 33, 36, 37, 40, 60, 71 e 88kD proteins also were identificated by sera. Quantitative immunologic recognizing study showed closer relation between positive clinical history and recognizing of allergens from of L vannamei than between theses with positive results in commercials kits. News investigations are needed to clear the relations between clinical manifestations and allergens recognizing of this shrimp species and another ingested in Bahia, to allow more specifics diagnostics