Immunohistochemistry study of the p53 protein in the penis condylomatose lesion.
In the last years the interest in human papilloma-virus (HPV) has grown due to an accumulation of evidence of its oncogenic potential, mainly in the anogenital tract. There are about hundred types of HPV, however only 40 generate lesions in the anogenital tract. The purpose of this study is to demonstrate the immunohistochemistry expression of the p53 protein in the penis condylomatose lesions in accompanied patients at the Dermatology Sector of the Service of Santa Casa da Misericórdia of Rio de Janeiro. Two articles have been written. A bibliographical review of the epidemiological and clinical manifestations of the infection by the HPV has been compiled in the first article, and in the second article the immunohistochemistry expression of the p53 protein in the penis condylomatose lesion has been evaluated. It has been demonstrated in the first article that the HPV epidemic has increased in last decades, and nowadays it can be considered as the most prevalent transmittable sexual disease, although the clinical manifestation of the infection (condylomatose) manifests itself in only 1% of the infected population, and that the greatest problem would be related to the latent or subclinical infections that might affect great part of the sexually-active population being involved in the carcinogenic process. In spite of the importance of the epidemiological and clinical manifestations in the HPV infection, the article has also evaluated other subjective problems which are impossible to be identified by laboratory methods: the social and psychological complications of the HPV infection. The second article has evaluated the expression of p53 protein in the penis condylomatose lesions. Three patients with a clinical diagnosis of condylomata acuminata have been selected, and a biopsys of the condylomatose lesions has been performed. The three patients? lesions have had a histopathological diagnosis of a low HPV infection with positive expression of the p53 protein. All cases have demonstrated epithelial hiperplasia and koiliocytosis. Two cases have demonstrated parakeratosis and only one case has demonstrated hiperkeratosis. The p53 protein expression was positive in all layers of the infected epithelium, and in one patient a strong expression concentated in the basal layer has been observed. The p53 protein expression has not demonstrated as being an efficient prognostic marker in HPV oncogenesis, as it is found to be positive in low degree lesions. Therefore, this work arrives to the conclusion that the expression of p53 protein is present within the HPV infections and cannot be related to the clinical presentation of the lesion and neither to the epithelial histopathological alteration.
Advisor:Gerson Cotta-Pereira; José Augusto da Costa Nery
School:Faculdades Oswaldo Cruz
Source Type:Master's Thesis
Tumor Suppressor Protein p53
Sexually Transmitted Diseases
Date of Publication:11/28/2006