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Identification, classification and notation of analogous enzyme in Trypanosoma Identification, classification and notation of analogous enzyme in Trypanosoma

by Ramos Guimarães, Ana Carolina

Abstract (Summary)
Enzymes are proteins capable of catalyzing chemical reactions. Analogous enzymes are alternative forms of a determined enzymatic function, and are interesting subjects for the development of studies about the evolution of genes and metabolic pathways. Analogous enzymes also represent potential drug targets, since the criteria for searching and selecting these targets are normally based in the specificity of enzymatic functions and not in the evolutionary origin of the different forms of a determined enzyme. In this work, we implemented a tool (AnEnp) capable of clustering protein sequences and visualizing the results; it also carries out similarity searches via BLAST and/or HMMER and reconstructs metabolic maps. With this tool it was possible to analyze data about the presence/absence of enzymatic functions in the genomes of Trypanosoma cruzi, Trypanosoma brucei and Leishmania major. These data were compiled, stored and analyzed. In addition, the variability of the metabolic pathways was studied, taking into account the existence of analogy events between trypanosomatids and the human being. A list of possible targets for development of new drugs was produced. Enzymes are proteins capable of catalyzing chemical reactions. Analogous enzymes are alternative forms of a determined enzymatic function, and are interesting subjects for the development of studies about the evolution of genes and metabolic pathways. Analogous enzymes also represent potential drug targets, since the criteria for searching and selecting these targets are normally based in the specificity of enzymatic functions and not in the evolutionary origin of the different forms of a determined enzyme. In this work, we implemented a tool (AnEnp) capable of clustering protein sequences and visualizing the results; it also carries out similarity searches via BLAST and/or HMMER and reconstructs metabolic maps. With this tool it was possible to analyze data about the presence/absence of enzymatic functions in the genomes of Trypanosoma cruzi, Trypanosoma brucei and Leishmania major. These data were compiled, stored and analyzed. In addition, the variability of the metabolic pathways was studied, taking into account the existence of analogy events between trypanosomatids and the human being. A list of possible targets for development of new drugs was produced.
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:Antônio Basílio de Miranda; Wim Maurits Sylvain Degrave

School:Faculdades Oswaldo Cruz

School Location:Brazil

Source Type:Master's Thesis

Keywords:trypanosoma analogous enzyme

ISBN:

Date of Publication:06/29/2006

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