Human testis angiotensin-converting enzyme: Crystal structure of a glycosylation mutant and investigation of a putative hinge-mechanism by normal mode analysis.

by Watermeyer, Jean Margaret

Abstract (Summary)
Human angiotensin-converting enzyme (ACE) is a key enzyme in the regulation of blood pressure via the renin-angiotensin and kallikrein-kinin systems. A number of orally active drugs have been developed over the years that target somatic ACE, for the treatment of hypertension, myocardial infarction and congestive heart failure. Protein structural information about ACE is an important key for the understanding of the mechanism and substrate-specificity of the enzyme. However, this information has only begun to be elucidated in the past year, with the solution of crystal structures of human testis ACE (tACE), and homologues Drosophila AnCE and human ACE2. tACE is identical to the C-terminal domain of somatic ACE, which consists of two homologous domains, each having a slightly different substrate-specificity. This thesis describes the purification, crystallisation and X-ray crystal structure-determination of a glycosylation-deficient mutant of tACE, tACEG1,3, to 2.9 Å
Bibliographical Information:


School:University of the Western Cape/Universiteit van Wes-Kaapland

School Location:South Africa

Source Type:Master's Thesis

Keywords:angiotensin converting enzyme glycosides


Date of Publication:01/01/2004

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