High-resolution fluorescence spectroscopy in immunoanalysis /
Abstract (Summary)The work presented in this dissertation combines highly sensitive and selective fluorescence line-narrowing spectroscopy (FLNS) detection with various modes of immunoanalytical techniques. It has been shown that FLNS is capable of directly probing molecules immunocomplexed with antibodies, eliminating analytical ambiguities that may arise from interferences that accompany traditional immunochemical techniques. Moreover, the utilization of highly cross-reactive antibodies for highly specific analyte determination has been demonstrated. Finally, we demonstrate the first example of the spectral resolution of diastereomeric analytes based on their interaction with a cross-reactive antibody.
School:Iowa State University
School Location:USA - Iowa
Source Type:Master's Thesis
Date of Publication: