Functional genomics in fish: towards understanding stress and immune responses at a molecular level
The main results of the present thesis can be integrated to a better understanding the stress and the immune responses in fish at a transcriptional level. The application of functional genomic tools, which encloses from using simple PCR analysis to more modern, sophisticate and fashionable microarray technique, allowed us to identified transcriptional regulations of certain set of genes which are enhanced or repressed under stress conditions. Our findings contribute to increase knowledge of molecular mechanism involved in coping the stress and immune responses in fish and provides a better understanding of fish physiology when fish health is threatened. Furthermore, thesis results may be interesting for aquaculture which looks for good biomolecular markers that may improve fish production and fish quality. The isolation, characterization and gene expression study with further microarray analysis of the enolase gene, allowed us to describe enolase as a possible biomolecular marker to determine fish welfare. The in situ hybridization study of the hematopoietic transcription factor PU.1, contributed to amplify the knowledge of the development of the fish immune system. Throughout this thesis, DNA sequences and mRNA expression levels of several genes studied, have contributed to enlarged genomic fish database.
In summary, this thesis described from a transcriptional level, gene expression and molecular mechanisms activated or repressed when fish welfare is threatened and contributes to a better understanding of transcriptiomic mechanisms required to cope with the stress.
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Advisor:Tort, Lluís; MacKenzie, Simon
School:Universitat Autónoma de Barcelona
Source Type:Master's Thesis
Keywords:408 departament de biologia cel lular i fisiologia
Date of Publication:07/10/2006