Expression strategies for plant-based production of a vaccine adjuvant
The specific goal of this research was to produce ricin in tobacco as adjuvant for the vaccine. Vectors were constructed that fused the ricin coding sequence to different plant promoters and transgenic tobacco plants were generated by transformation with Agrobacterium tumefaciens. The levels of expression in these transgenic plants were tested using immunoblot assays. Southern blot analysis was performed for the highest expressors of each construct. The enzymatic activity of the tobacco-synthesized ricin was shown using a protein translation inhibition assay. Expression of ricin was also confirmed using transient transformation of hairy root cultures. Future experiments will address the practical use of the tobacco-synthesized ricin as adjuvant, as well as the expression of the ricin B subunit fused to a protective antigen of Entamoeba histolytica in tobacco as edible vaccine.
Advisor:Dr. Cynthia Denbow; Brenda S. J. Winkel; Dr. C. Cramer
School Location:USA - Virginia
Source Type:Master's Thesis
Keywords:plant pathology physiology and weed science
Date of Publication:04/26/2000