Estudo morfológico e morfométrico do efeito de filmes bioativos decolágeno contendo própolis vermelha sobre o processo de reparocicatricial de queimaduras de terceiro grau em ratos

by de Almeida, Enrik Barbosa

Abstract (Summary)
This research aimed at evaluating morphologically the third-degree burn healingprocess treated with collagen biofilms containing hydroalcoholic extrat of redpropolis. Thus, type I collagen-based films extracted from bovine tendon wereprepared containing hydroalcoholic extract of 0,1% red propolis. Subsequently,third-degree burn wounds of 1cm2 were done in the dorsum of 30 Wistar rats,that were distributed in six groups (n=5): G1 and G2 ? animals with notreatment were sacrificed in 14 and 21days; G2 and G4 ? animals treated withcollagen membranes sacrificed in 14 and 21days; G3 and G6 ? animals treatedwith collagen membrane containing red propolis sacrificed in 14 and 21days.The specimens removed were formalin-fixed, and paraffin-embedded andhistological sections were stained in HE. On the fourteenth day, theinflammatory reaction was less intense in G3, and the leukocytic profile showedremarkable plasma cells infiltratation. On the twenty-first day, there wasunequivocal reduction of the inflammation, which was predominantly composedof plasma cells in all the groups. The mean of blood vessels was significantlyhigher in both groups treated with propolis-containing collagen films sacrificedat 14 and 21 days (plt;0.05). Regarding the vascular perimeter, there wassignificant differences among all the groups at both 14 and 21 days, and thegroups G3 and G6 presented the less means of blood vessels perimeters. Themean of mast cells was significantly lesser in G1 than in G2 and G3 (plt;0.05),but there was no difference on the twenty-first day. Fibroplasia was moreintense in the groups treated with collagen films containing red propolis extract(G3 and G6), and less expressive in the control ones (G1 and G4). Besides aclear tendency to better epithelization was observed in both groups treated withpropolis-containing collagen-based films sacrificed at both 14 and 21 days,although this difference was not statistically significant. It was concluded thatthe use of reconstituted bovine type-I collagen films containing 0.1%hydroalcoholic extract of red propolis provided some improvements in the burnhealing process in rats
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:Nicodemos Teles Pontes Filho

School:Universidade Federal de Pernambuco

School Location:Brazil

Source Type:Master's Thesis

Keywords:Collagen Wound healing Burn wounds


Date of Publication:08/18/2008

© 2009 All Rights Reserved.