Employing Microvoluminal Phlebotomization Method to Scrutinize the Immunity of Grouper Vaccinated with Polyvalent Vaccines

by Chen, Hsin-Hong

Abstract (Summary)
The grouper is a high-value fish in Taiwan. However, nervous necrosis virus, which contains two single strand RNAs and doesn¡¦t have an envelope, causes groupers die in early stages of development. It economically impacts on the aquaculture of this marine fish. Vaccination is one of the best methods to prevent viral diseases. Virus-like particles (VLPs) were used for studying the ability and efficiency of producing antibodies against dragon grouper nervous necrosis virus. Different injection dosages and injection frequency of VLPs were performed on Epinephelus lanceolatus. The anti-sera of vaccinated fish were acquired by microvoluminal phlebotomization method and analyzed by antigen-capture dot blotting with ECL detections, which are the best choices for qualitative and quantitative assays. The signal of antibodies in the vaccinated fish was detected every week after primary immunization. The antibody signal reached 8.4x106 U in one month when the dragon groupers were injected with 10 µg of VLPs, but giving additional injection of VLPs didn¡¦t increase production of antibodies after one month. Moreover, dragon groupers that were injected with 50 µg and 100 µg of VLPs generated antibody signals up to 7.2x106 U and 6.7x106 U, respectively. The antibody signal can remain at a higher level in the presence of high dosage injection for at least three weeks. Furthermore, a polyvalent vaccine that included killed bacteria was applied in field to test dragon grouper immune response. The results not only support the data that dragon grouper boots antibody production within a short period after immunization, but also demostrates that the production of antibody by dragon grouper against virus and bacteria can be monitored by the microvoluminal phlebotomization method.
Bibliographical Information:

Advisor:Chan-Shing Lin; Jong-Kang Liu; Chi-Hsin Hsu

School:National Sun Yat-Sen University

School Location:China - Taiwan

Source Type:Master's Thesis

Keywords:dragon grouper vlps


Date of Publication:05/21/2007

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