Effects of pathogen on blood and serum chemistry of grouper
The purpose of this study was for building the data on blood and serum chemistry of grouper ‚ and expect these data can be helpful to know the fish condition in early stages‚ in order to remedy as soon as possible and decrease the loss that caused of fish disease. Three experiments were conducted to establish the data on blood and serum chemistry of grouper. In the first experiment‚ the clinical chemistry including red and white blood cell counts‚ Glutamic oxalacetic transaminase (GOT)‚ Glutamic pyruvic transaminase (GPT)‚ total protein‚ albumin and blood ion of Na‚ K and Ca were built in different size of healthy orange spotted grouper¡]Epinephelus coioides¡^. The results showed that the white blood cell counts was divided into two major parts 70-90´103 cell/ml and 110-140´103 cell/ml. Red blood cell counts was landed on 1.85-3.21´106 cell/ml. The range of GOT and GPT were 0-45 IU/L and 0-350 IU/L. Total protein and albumin were 3.8-5.6 and 0.7-1.6 g/dL. In the second experiment‚ the clinical chemistry of orange spotted grouper after infected by dactylogurus‚ trichodinla‚ nervous necrosis virus (NNV) and NNV with Amyloodinium ocellatum were collected and compared with normal orange spotted grouper. The white blood cell counts and the concentration of K+ in the infected NNV and dactylogurus of fish were significantly higher (P<0.05) than normal fish‚ while the granulocyte in infected fish was significantly lower (P<0.05) than normal fish. In the dactylogurus and trichodinla infections of fish‚ the concentration of Ca2+ was significantly higher (P<0.05) than normal fish. In the third experiment‚ the size of monocytes and neutrophils of brown marbled grouper¡]E. fuscoguttatus¡^ were grown in 8 hours and 16 hours‚ respectively‚ after challenged with Photobacterium damselae subsp. damselae‚ and the eosinophils size of challenge group fish bigger (P<0.05) than control group fish after challenged for 16 hours.
Advisor:Tse-min Lee; Cheng-fang Chang; Jung-pin Hsu; Su-lean Chang
School:National Sun Yat-Sen University
School Location:China - Taiwan
Source Type:Master's Thesis
Keywords:photobacterium damselae subsp serum blood grouper
Date of Publication:09/15/2006