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Effects of diets, antimicrobials and minerals on the revalence and antimicrobial susceptibility of fecal bacteria in feedlot cattle

by Jacob, Megan E

Abstract (Summary)
Antimicrobials are included in finishing cattle diets for growth promotion, feed

efficiency, and protection against liver abscesses. The inclusion of in-feed antimicrobials at or

below therapeutic concentrations may provide a selective pressure for antimicrobial resistant

microorganisms. Additionally, heavy metals such as copper and zinc may be included in cattle

diets because of growth-promoting effects. Heavy metal resistance genes are on transferable

plasmids that also contain antimicrobial resistance genes. The objectives of this research were to

1) determine the prevalence of food-borne pathogens, Salmonella and E. coli O157, in cattle fed

diets with or without monensin and tylosin and 0 or 25% wet corn distiller's grains (WDGS), 2)

determine the prevalence of food-borne pathogens in cattle fed elevated concentrations of copper

and zinc 3) evaluate the effect of antimicrobials on antimicrobial susceptibility of food-borne

pathogens and commensal fecal bacteria, and 4) determine a possible association between in-feed

antimicrobials and the concentration of antimicrobial resistance genes in the feces of cattle.

Inclusion of 25% WDGS was associated with a higher prevalence of E. coli O157 on one of two

sample collection days; however, there was no association between the use of monensin and

tylosin, or copper and zinc on the prevalence of food-borne pathogens. Including monensin and

tylosin in cattle diets was associated with an increased resistance of enterococci to macrolides,

but was not related to concentration of the common macrolide resistance gene, ermB. In cattle

fed diets with copper and/or zinc, no differences were observed in antimicrobial susceptibility or

the concentration of antimicrobial resistance genes. In conclusion, results indicate that including

growth-promoting antimicrobials in cattle diets at below therapeutic concentrations only

limitedly impacted antimicrobial susceptibility and concentration of fecal antimicrobial

resistance genes; however, this research encompassed only a select number of microorganisms.

The positive association between WDGS and E. coli O157 prevalence in cattle has important

implications for food safety, and warrants further investigation.

Bibliographical Information:

Advisor:

School:Kansas State University

School Location:USA - Kansas

Source Type:Master's Thesis

Keywords:antimicrobial feed additives susceptibility distiller s grains cattle heavy metal resistance food borne pathogens biology microbiology 0410 veterinary science 0778

ISBN:

Date of Publication:01/01/2007

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