The Effects of Cardiotonic Steroids on Dermal Collagen Synthesis and Wound Healing

by El-Okdi, Nasser Samir

Abstract (Summary)
We previously reported that cardiotonic steroids stimulate collagen synthesis by cardiac fibroblasts in a process that involves signaling through the Na/K-ATPase pathway. In this study, we examined the effect of cardiotonic steroids on dermal fibroblasts collagen synthesis and on wound healing. Increased collagen expression by human dermal fibroblasts was noted in response to the cardiotonic steroid, marinobufagenin, in a dose and time dependent-fashion. Next, we examined the effect of digoxin, ouabain and marinobufagenin on the rate of wound closure in an in vitro model where human dermal fibroblasts cultures were wounded with a pipette tip and monitored by digital microscopy. Finally, we administered digoxin in an in vivo wound healing model, using olive oil as a vector. This application significantly accelerated in vivo wound healing in rats wounded with an 8 mm biopsy cut. Increased collagen accumulation was noted 9 days after wounding (both p < 0.01). These data suggest that cardiotonic steroids induce increases in collagen synthesis by dermal fibroblasts, and accelerate wound healing.
Bibliographical Information:


School:University of Toledo Health Science Campus

School Location:USA - Ohio

Source Type:Master's Thesis

Keywords:collagen fibroblast cardiotonic steroids wound healing na k atpase


Date of Publication:01/01/2008

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