The Effects of Cardiotonic Steroids on Dermal Collagen Synthesis and Wound Healing
We previously reported that cardiotonic steroids stimulate collagen synthesis by cardiac fibroblasts in a process that involves signaling through the Na/K-ATPase pathway. In this study, we examined the effect of cardiotonic steroids on dermal fibroblasts collagen synthesis and on wound healing. Increased collagen expression by human dermal fibroblasts was noted in response to the cardiotonic steroid, marinobufagenin, in a dose and time dependent-fashion. Next, we examined the effect of digoxin, ouabain and marinobufagenin on the rate of wound closure in an in vitro model where human dermal fibroblasts cultures were wounded with a pipette tip and monitored by digital microscopy. Finally, we administered digoxin in an in vivo wound healing model, using olive oil as a vector. This application significantly accelerated in vivo wound healing in rats wounded with an 8 mm biopsy cut. Increased collagen accumulation was noted 9 days after wounding (both p < 0.01). These data suggest that cardiotonic steroids induce increases in collagen synthesis by dermal fibroblasts, and accelerate wound healing.
School:University of Toledo Health Science Campus
School Location:USA - Ohio
Source Type:Master's Thesis
Keywords:collagen fibroblast cardiotonic steroids wound healing na k atpase
Date of Publication:01/01/2008