Efecto del ejercicio físico agotador sobre el estrés oxidativo asociado al envejecimiento
Free radical theory of aging is the most accepted and tested theory to explain the aging process. According to this theory, the progressive and cumulative harmful effects of free radicals along life causes aging and death. Free radicals are also involved in exercise. They increase when performing an exhausting exercise, leading to oxidative stress. With the aim of assessing the role of xanthine oxidoreductase as a free radical source in the aging process and his contribution to oxidative stress that occurs in exhausting exercise, we have studied the effect of exhausting exercise in young and old male wistar rats. We measured skeletal muscle damage parameters in plasma, oxidative stress in blood, liver and skeletal muscle, xanthine oxidoreductase activity and expression in plasma, liver, skeletal muscle and aorta, and superoxide production in aortic rings. Our results show that aging is associated to blood, liver and skeletal muscle oxidative stress, as well as an increase in xanthine oxidase activity and expression in plasma, skeletal muscle, and aorta, and also an increase in superoxide production in aorta. Exhausting exercise leads to an increase of skeletal muscle damage parameters in plasma and to an increase of oxidative stress in blood, liver and skeletal muscle either in young or old animals, as well as to an increased xanthine oxidase activity in plasma of young animals.
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Advisor:Viña Ribes, José; Pallardó Calatayud, Federico V.
School:Universitat de València
Source Type:Master's Thesis
Date of Publication:09/25/2003