ESTUDOS GENÉTICO-FISIOLÓGICOS DA OSMOREGULAÇÃO NO LINGUADO Paralichthys orbignyanus. ESTUDOS GENÉTICO-FISIOLÓGICOS DA OSMOREGULAÇÃO NO LINGUADO Paralichthys orbignyanus.

by Meier, Karina Maria

The objective of this study was to investigate the gill Na+, K+-ATPase activity, plasma ionic (Na+, K+ e Cl-) concentration, histological modifications on gill, kidney and oesophagus; and gene expression of growth hormone (GH) and your receptor (GHR) after transfer of flounder Paralichthys orbignyanus to seawater. The isolation, sequence and expression of GH and partial sequence of GHR cDNA were obtained using the reverse transcription-polymerase chain reaction (RT-PCR). After some days exposure to different salinities, no changes in gill Na+, K+-ATPase activity and histology were detected. Fishes reared in seawater (SW) displayed increased in plasma ionic (Na+ e K+) concentration after 6 hours, and in Cl- after 3 hours. Theexpression level of growth hormone gene in the seawater-reared fish was higher than that of brackish water, and the mRNA growth hormone receptor was detected in the gill of this flounder. These results suggest the possible involvement of GH in SW adaptation in this species, and probably the marine origin of the flounder explains your high tolerance to salinity changes over its isomotic point without morfological and enzyme activity modifications. The objective of this study was to investigate the gill Na+, K+-ATPase activity, plasma ionic (Na+, K+ e Cl-) concentration, histological modifications on gill, kidney and oesophagus; and gene expression of growth hormone (GH) and your receptor (GHR) after transfer of flounder Paralichthys orbignyanus to seawater. The isolation, sequence and expression of GH and partial sequence of GHR cDNA were obtained using the reverse transcription-polymerase chain reaction (RT-PCR). After some days exposure to different salinities, no changes in gill Na+, K+-ATPase activity and histology were detected. Fishes reared in seawater (SW) displayed increased in plasma ionic (Na+ e K+) concentration after 6 hours, and in Cl- after 3 hours. Theexpression level of growth hormone gene in the seawater-reared fish was higher than that of brackish water, and the mRNA growth hormone receptor was detected in the gill of this flounder. These results suggest the possible involvement of GH in SW adaptation in this species, and probably the marine origin of the flounder explains your high tolerance to salinity changes over its isomotic point without morfological and enzyme activity modifications.