Differential Responsiveness of Early and Late Passage Endothelial Cells to Shear Stress
I hypothesize that shear stress helps maintain the quiescent phenotype of vascular endothelium. I also hypothesize that changes in levels of shear stress facilitate alternations in endothelial cell activity consistent with an activated phenotype and the development of atherosclerotic vascular disease.
Purpose #1: To assess the effects of aging on EC proliferation induced by SS
Hypothesis: Orbital SS stimulates EC proliferation. Aged cells are less responsive to orbital SS and show less proliferation.
Purpose #2: To assess the effects of aging on EC survival and apoptosis
Hypothesis: Versus younger cells, aged cells have increased rates of apoptosis.
Purpose #3: To assess the effects of aging on SS induced SMC chemotaxis
Hypothesis: Aged EC are less responsive to orbital SS and induce less SMC chemotaxis versus younger cells.
Purpose #4: To assess the effects of aging on Akt pathway activation with SS
Hypothesis: Orbital SS activates EC leading to phosphorylation of Akt. Versus younger cells, aged cells are less responsive to orbital SS and show less stress induced phosphorylation of Akt.
School Location:USA - Connecticut
Source Type:Master's Thesis
Keywords:endothelial cells stress atherosclerosis aging apoptosis
Date of Publication:11/10/2006