AVALIAÇÃO DE ATIVIDADESFARMACOLÓGICAS DEDIFERENTES ESPÉCIES DELYCHNOPHORA UTILIZADASPELA POPULAÇÃO
The Lychnophora genus species are native plants from Brazil, popularly knownas ?arnica? and used in the folk medicine as analgesic and anti-inflammatory. Theantinociceptive activity of the etanolic extracts of six species of Lychnophora wereevaluated in the hot-plate test and in the acetic acid-induced writhing test and the antiinflammatorytopical activity were evaluated in the carrageenan-induced paw oedematest. In the hot-plate test the etanolic extracts of L. pinaster (0.75 g/kg) and L. ericoides(1.50 g/kg), significantly increased the variation of the latency time for licking of thepaws. The extracts of L. passerina, L. candelabrum and L. pinaster in the dose0.75 g/kg and of L. ericoides and L. trichocarpha in both doses evaluated (0.75 and1.50 g/kg) significantly decreased the number of writhes induced by acetic-acid. Thetopical administration of L. pinaster and L. trichocarpha in both concentrationsevaluated (5 and 10%) and of L. passerina and L. candelabrum 10%, significantlyreduced the paw oedema measured 3 h after the subplantar injection of carrageenan0.1%, evidencing for the first time the anti-inflammatory activity of the topicaladministration of Lychnophora species. The method of measurement of the paw oedemausing a pakimeter were validated and showed that the pakimeter is so effective how theplethysmometer to measure the paw oedema induced by carrageenan.The effects of the extracts of L. ericoides and L. trichocarpha were evaluatedbefore, 1, 2, 4 and 24 h after the oral administration, in the open-field test, that evaluatethe locomotor and exploratory activities and in the rota-rod and traction tests, wichevaluate the motor coordination and the muscular tone of the animals, respectively. Inthe open-field test, the extract of L. ericoides (1.50 g/kg) in 1 h and the extract ofL. trichocarpha in the dose 0.75 g/kg in 2 h and in the dose 1.50 g/kg in the times 1, 2and 4 h induced to significant inhibition of the spontaneous locomotor activity of theanimals evidenced by the reduction of the number of squares crossed in 5 min. Theextracts of L. ericoides (0.75 g/kg) in 24 h and of L. trichocarpha in the dose 0.75 g/kgin 2 h and in the dose 1.50 g/kg in the times 1, 2 and 4 h induced to the decrease of theexploratory behavior of the animals, evidenced by the significant decrease of thenumber of times that the animal raises in 5 min. The decrease of the spontaneouslocomotion and the exploratory behavior induced by the administration of these extactsAbstractcompared to the control group were indicative of sedative effect. The extracts ofL. ericoides and L. trichocarpha in both doses (0.75 and 1.50 g/kg) and in all timesevaluated did not induce significant modification of the motor coordination and themuscular tone of the animals.Beyond the evaluation of the activity on the SNC, in the present study to betterestablish the therapeutical security of the L. ericoides ethanolic extract, the specie mostused by the population, its cardiovascular activity was evaluate from 90 to 120 min afterits administration by oral route. The results demonstrated that the L. ericoides extract inthe dose 1.50 g/kg increased the systolic and diastolic arterial pressure and the heart rateof the animals.The present work demonstred comparatively the antinociceptive and antiinflammatoryactivities of six species of Lychnophora, the sedative activity ofL. ericoides and L. trichocarpha species and the hypertensive and cronotropic positiveactivities of L. ericoides in the higher dose evaluated, giving cientific knowledge for therational and directed use of the species studied in the folk medicine.
Advisor:Andréa Grabe Guimarães; Raquel do Pilar Machado
School:Universidade Federal de Ouro Prêto
Source Type:Master's Thesis
Keywords:1 agentes antiinflamatórios teses 2 cardiovasculares 3 farmacologia bioquimica
Date of Publication:05/11/2007