DETERMINATION OF in vitro DRUG RELEASE FROM WOUND DRESSINGS THROUGH AN ARTIFICAL WOUND MODEL
Most wounds to the skin will cause leakage of blood from damaged blood vessels. A stable fibrin matrix is fundamental in wound edge adhesion and wound healing. For topically applied wound treatments to be effective they must penetrate this fibrin clot. Therefore, the objective of the study was to use a fibrin biopolymer as a model wound to investigate drug diffusion from wound dressings. Four compounds known to play an important role in wound healing were utilized. There were apigenin, vitamin E, chlorhexidine digluconate (CHDG) and benzalkonium chloride (BAC). Initial experiments demonstrated that no detectable amounts of apigenin and benzalkonium chloride were found in the provided burn dressings and bandages. As a result, no further attempts were made to study the release of these two compounds from the delivery systems. Release studies from delivery systems containing the other two compounds through the fibrin sealant were continued. The experiments demonstrated that the release data (Q, Q%) from burn dressings containing 4, 6, 10% vitamin E plotted versus time ½ and versus time had linear coefficient from 0.92 to 0.96 and from 0.94 to 0.97. This indicates that the release of vitamin E from burn dressing through fibrin sealant may be matrix controlled or membrane controlled, and the experiment could not distinguish between the two models. There was a linear relationship between the slope ^2 of Q- t ½ and initial amount of vitamin E in burn dressing. The in vitro release of chlorhexidine digluconate from sterile and non-sterile bandages was compared. There was no significant difference between these two groups at any cumulative release time interval. The release appears to be a matrix-controlled. The results indicate that fibrin sealant may be potentially a useful model in evaluating release of drugs utilized in treatment of superficial wounds.
School:University of Cincinnati
School Location:USA - Ohio
Source Type:Master's Thesis
Keywords:fibrin clot diffusion vitamin e chlorhexidine digluconate membrane
Date of Publication:01/01/2002