CULTURE-BASED MICROFLUIDIC DEVICE FOR RAPID DETECTION OF MYCOBACTERIA
Abstract (Summary)This thesis describes the development of a culture-based microfluidic device for detecting mycobacteria in environmental samples. This microdevice, made in cyclic olefin copolymer (COC), uses the paraffinophilic nature of mycobacteria to rapidly detect and differentiate them from non-target microorganisms. New methods of depositing and patterning paraffin were developed to fabricate the prototype device. Paraffin films 3-7 µm thick were successfully deposited and patterned on COC surface by spin coating and lift-off techniques. COC based microfluidic chips were fabricated by hot embossing with PDMS masters. The final device with patterned paraffin film and microfluidic channels was packaged with adhesive bonding. Microfluidic devices were experimentally tested to demonstrate rapid detection of mycobacteria in pure cultures and environmental samples collected from a municipal sewage treatment plant. Target microorganisms, such as G. amarae and T. pauromycobacteriuim were successfully detected on the paraffin patterns whereas only cell debris was present on surfaces with the non-target microorganism, such as E. coli. The successful demonstration of the culture-based biochip presents an alternative approach for developing new technology to track microorganisms in complex environmental samples.
School:University of Cincinnati
School Location:USA - Ohio
Source Type:Master's Thesis
Date of Publication:01/01/2004