Contribution of potassium channels to myogenic response in skeletal muscle arterioles [electronic resource] : effects of age and fiber type /
In isolated skeletal muscle arterioles, increasing transmural pressure causes an increase in constriction. This active myogenic response varies with age and fiber type. Increased transmural pressure activates both Ca²?-activated (Kca) potassium channels and voltage-dependent (Kv) potassium channels; these channels have a role in the negative feedback pathways that modulate depolarization and myogenic constriction. We tested the hypothesis that increased Kca channel and Kv channel activity contribute to reduced myogenic responsiveness in skeletal muscle arterioles of aged rats. 1A arterioles were isolated from soleus, an oxidative muscle, and superficial gastrocnemius, a glycolytic muscle, of young (4 mos) and aged (24 mos) Fischer 344 rats. Myogenic responses were assessed by increasing intraluminal pressure (0-140 cm H?O) in increments of 20cm H?O.Vasoconstrictor response were determined in response to increasing concentrations of the Kca channel blocker, charybdotoxin (CTX; 10?¹?- to 10?? M) and the Kv channel blocker, 4-Aminopyridine (4-AP; 10?? to 10?² M). To determine the role of potassium channels in modulating the myogenic response, cannulated arterioles from soleus and gastrocnemius were incubated with CTX (50 nM) and 4-AP (5mM) for 15 minutes prior to evaluation of the myogenic response. Increased Kv channel activity contributes to reduced myogenic constriction in soleus and gastrocnemius muscle arterioles from aged rats. In soleus muscle arterioles, Kca channel activity opposes myogenic tone in young but not old rats. In gastrocnemius muscle arterioles, treatment with CTX did not eliminate age-related differences in the myogenic response, and the Kca channel contribution to myogenic tone was, in fact, greater arterioles from young as compared to old rats. Kv channels contribute to greater myogenic constriction in soleus arterioles, Kca channels appear to be more active in gastrocnemius muscle arterioles as compared to soleus muscle arterioles.Therefore Kv and Kca channels are tonically active in skeletal muscle arterioles, contributing to a hyperpolarizing force that opposes myogenic constriction. Furthermore, increased Kv channel activity contributes to the age-related reduction of myogenic constriction in soleus and gastrocnemius muscle arterioles.
School:Texas A&M International University
School Location:USA - Texas
Source Type:Master's Thesis
Keywords:major kinesiology potassium channels 4 ap myogenic response ctx
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