Comparison of Polymerase Chain Reaction with Microscopic Examination to detect Trypanosoma cruzi in Triatoma infestans digestive tract samples

by Almeida Braz, Lucia Maria

Abstract (Summary)
Detection of T. cruzi in T. infestans digestive tract samples was compared by PCR and microscopic examination (ME) on the 1st to 5th, 10th, 15th, 20th, 25th, 30th and 60th day after feeding in Balb/C mice infected by T. cruzi, and divided in two groups: with high parasitemia and with low parasitemia. PCR presented vantages to the Chagas' disease diagnosis when compared with EM in 10 of 11 times in high parasitemia, and in all times in low parasitemia. Earlier diagnosis in low parasitemia model is of clinical relevance since may influence prognosis of immunosupressed patients in reactivation of Chagas' disease
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:Thelma Suely Okay; Ulysses Doria Filho; AbĂ­lio Augusto Fragata Filho; Vicente Amato Netto; Sonia Regina Testa da Silva Ramos; Thelma Suely Okay

School:Universidade de São Paulo

School Location:Brazil

Source Type:Master's Thesis

Keywords:Chagas disease/diagnosis Gastrointestinal contents Microscopy Polymerase chain reaction Triatoma Trypanosoma cruzi


Date of Publication:06/27/2006

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