Cloning and Expression of a Diagnostic Antigen for Invasive Amoebiasis
Immunoblot analysis of the crude E. histolytica extract indicated the presence of several antigenic proteins. One of the common antigenic proteins recognized by the individual patients' sera had a molecular weight of 160.170 kDa Sera of five patienta with high titers of E. histotytica-specific IgG antibodies were used to prepare a serum pool. This pooled serum was used for immunoscreening of an E. histolyitca cDNA expression library prepared in the phage vector l ZAP-II. A strongly immunoreactive phage done was identified, from which he recombinant phagemid was released by in vivo excision for characterization of the cDNA insert.
Advisor:Subba Rao, P V
School:Indian Institute of Science
Source Type:Master's Thesis
Keywords:limnology invasive amoebiasis cloning e histolytica
Date of Publication:08/01/1994