Characterization of transgenic tobacco plants (Nicotiana tabacum L.) which express the pea lhcb1*2 gene, upon chloroplast development and assembly of the photossystem II.

by Cordeiro, Raqueline Cunha

Abstract (Summary)
The vegetal production is strictly dependent on the photosynthetic process. Techniques of molecular biology and genetic transformation of plants brought good perspectives for the alteration of the photosynthetic metabolism. Transgenic tobacco plants (Nicotiana tabacum, L.) which express the chimeric pea Lhcb1*2 gene were pbtained and presenta series of alterations on development and photosynthetic metabolism in relation to the wild type. Previous analysis have demonstrated morphological, physiological, biochemical and adaptative changes that favour these transgenic lines in various conditions of culture. The aim of this work was to evaluate the impact of the expression of this gene in the plastid formation, of tobacco seedlings. Seeds were germinated and kept in darknes for seven days, and transferred to light. The seedlings were then collected after 0, 6, 18 and 120 hours of exposure to continuous ilumination. The plastidial development evaluated by light microscopy, showed an advanced chloroplast formation of the transgenic lines (TR1 and TR2) in relation to the wild type (WTSR1). The ultrastructural analysis of the plastids by electronic microscopy showed, indeed on advanced formation of mature chloroplasts in the transgenic lines. The Western blot analysis confirmed the presence of two specific proteins (CAB and D1), of the photosystem II. This fact implies that the assembly of the photosynthetic apparatus might occurs earlier in the transgenic lines, as well as the morphological and structural development of the plastids.
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:Carlos Alberto Labate; Maria Helena de Souza Goldman; Elliot Watanabe Kitajima; Carlos Alberto Labate

School:Universidade de São Paulo

School Location:Brazil

Source Type:Master's Thesis

Keywords: electronic microscopy photosynthetic metabolism plastídeos plastids tobacco transgenic plants


Date of Publication:11/18/2004

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