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Characterization of Marek's disease antigens in JMV-1 cell line

by Bhogle, Amrut Shridhar

Abstract (Summary)
JMV-1 cell-line, a Marek's disease virus transformed lymphoblastoid cell-line, could be adapted to grow in serum free medium conditions. The cell-line exudes into its growth medium a complex array of proteins ranging from high molecular weight to low molecular weight. JMV-1 cell-line inspite of its helper T-cell phenotype with the presence of Ia antigen on its surface did not produce any interleukin-2, interferon or macrophage activating factor. Marek's disease virus could not be isolated form the JMV-1 cell-line. An analysis of the genome of the JMV-1 cell-line indicated the presence of the entire Marek's disease virus genome. The genome had few copies of Bam HI fragment C, -E and -G while multiple copies of Bam HI fragments -D, -H and I$\sb2$ were observed. The ultrastructural study of JMV-1 did not reveal the presence of any Marek's disease virus particles in the nucleus. A viral inclusion like particle was observed in an isolated cell. JMV-1 cell-line produced Marek's disease virus specific antigens. These antigens could be precipitated by convalescent serum from Marek's disease virus infected birds. At least three species of antigens could be precipitated. The molecular weight of the proteins were 100000 d, 60,000 d and 50,000 d. JMV-1 cell-line supernatant imparts immunity against JMV lethal lethal lymphoblastoid transplant. The immunity is lost when JMV-1 supernatant is passed through anti-Marek's disease affinity column. The precipitated antigens failed to impart immunity against JMV. JMV-1 supernatant failed to induce antibodies in chicken nor did the supernatant induce cell-proliferation in-vitro.
Bibliographical Information:

Advisor:

School:University of Massachusetts Amherst

School Location:USA - Massachusetts

Source Type:Master's Thesis

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ISBN:

Date of Publication:01/01/1996

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