Biofilm Removal Using Bubbles and Sound Biofilm Removal Using Bubbles and Sound
To investigate the influence of sound on removal, a Ling oscillator was attached to the chamber, and was used simultaneously with and without a bubble stream. The acoustic intensity and the frequency were varied to determine if there was any effect on biofilm removal. Biofilm removal was also video taped.
The results of these experiments confirmed that biofilms are removed by a stream of bubbles. Removal of biofilm is a function of stream velocity, gas fraction, and median bubble diameter, but not of impingement angle. The results of the acoustic experiments show that sound does not affect the removal of biofilm under the conditions used in these experiments.
Mathematical models relating the removal of biofilm as a function of time were also developed from the data obtained from the video recording of the experiments.
Additional tests using acoustic waves to remove biofilm should be performed to determine if more intense sound can remove biofilm. The intensity of the sound used in these experiments was low and the time of exposure was only 5 sec. Additional tests that more closely simulate the conditions of the mouth during brushing, like adding a surfactant, would also provide more insight as to whether bubbles in a clinical setting would remove biofilm.
School:Brigham Young University
School Location:USA - Utah
Source Type:Master's Thesis
Keywords:biofilm bacteria streptococcus mutans bubbles sound acoustic waves angle
Date of Publication:06/23/2005