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Association of the blood biochemical index with CYP2E1 5¡¦flanking region Rsa I/Pst I gene polymorphism and alcohol consumption habit in Southern Taiwan aborigines

by Kuo, Chia-ling

Abstract (Summary)
The microsomal ethanol oxidizing system (MEOS) is involved in metabolism of alcohol in the liver, the major component of MEOS is cytochrome P4502E1 (CYP2E1). CYP2E1 gene polymorphisms that alter its functions may be associated with alcohol susceptibility in individual. A RsaI/PstI restriction fragment length polymorphism (RFLP) has been reported in the 5¡¦-flanking region of CYP2E1 gene. The rare mutant allele (c2 allele) that lacks the Rsa I restriction site, but can cut with Pst I has been found to be associated with higher transcriptional activity, mRNA expression, protein levels and enzyme activity than the commom wild type-c1 allele. CYP2E1-dependent oxidative stress on the pathogenesis of alcohol-induced liver injury. The recent epidemiological studies have point out that there are a high prevalence of alcohol consumption in aborigines of Southern taiwan. Dr.Bosron presented an drinks wine the behavior receives the environment and the genetic factor influence, this kind of difference also receives the racial influence to be really big. This study focus on the aborigines with high alcohol drinks rate to examine the possible effect on drinks habit and gene polymorphism of CYP2E1 RsaI/PstI to blood biochemicial index. The experimental result found that male aborigines drinker in blood pressure, triglyceride, HDL cholesterol, uric acid and AST value more than male aborigines non-drinker. However the values are in T.cholesterol, LDL cholesterol, creatinine less than non-drinker. The female aborigines drinker in blood pressure, HDL cholesterol, uric acid, creatinine and AST value more than female aborigines non-drinker. However the values are in T.cholesterol and LDL cholesterol less than non-drinker. Nevertheless, male aborigines drinker and female aborigines non-drinker did not achieved diversity of statistical in function of CYP2E1 gene polymorphism to biochemical index. Male aborigines non-drinkers have c1/c2+c2/c2 in CYP2E1 gene polymorphism compare to c1/c1 also have higher WHR (0.93¡Ó0.05 vs. 0.90¡Ó0.06, p = 0.035) and ALT (30.0¡Ó22.0 IU/L vs. 21.5¡Ó11.0 IU/L , p = 0.012). To summary, male aborigines who have c2 allele in CYP2E1 gene polymorphism may relate to obese and Liver dysfuction.
Bibliographical Information:

Advisor:Jiin-Tsuey Cheng; Yau-jiunn Lee; Chung-Lung Cho; Yo-ling Shiue

School:National Sun Yat-Sen University

School Location:China - Taiwan

Source Type:Master's Thesis

Keywords:southern taiwan aborigines cyp2e1gene polymorphism

ISBN:

Date of Publication:08/24/2005

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