Application of ultra high hydrostatic pressure for investigating the binding of flavor compounds to ß-lactoglobulin via headspace solid phase microextraction-gas chromatography
120
100
80
y
=
0.997x
-
5.5514
R2
=
0.9967
60
Po*a
40
20
0
0
20
40
60
80
100
120
-20
Ro*a/(1-a)
Figure
18.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
K’d
and
n
for
native
BLG
at
pH
5.0.
66
80 70
y
=
1.4244x
-
5.4289
R2
=
0.994
60 50 40
Po*a
30 20
67
10 0
0
10
20
30
40
50
60
-10
Ro*a/(1-a)
Figure
19.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
K’d
and
n
for
untreated
BLG
and
UHP-treated
(600
MPa)
BLG
with
0
min
holding
time
at
pH
5.0.
80 70 60
y
=
1.4151x
-
7.1145
R2
=
0.954
50 40 30
Po*a
20 10 0 -10
0
5
10
15
20
25
30
35
40
45
50
Ro*a/(1-a)
Figure
20.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
K’d
and
n
for
untreated
BLG
and
UHP-treated
(600
MPa)
BLG
with
4
min
holding
time
at
pH
5.0.
68
70 60 50 40
y
=
1.4706x
-
5.3086
R2
=
0.9816
30
Po*a
20 10 0 -10
0
5
10
15
20
25
30
35
40
45
50
Ro*a/(1-a)
Figure
21.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
K’d
and
n
for
UHP
treated
(600
MPa)
BLG
with
8
min
holding
time
at
pH
5.0.
69
80 70 60
y
=
1.6185x
-
11.583
R2
=
0.9996
50 40
Po*a
30 20 10 0
0
10
20
30
40
50
60
Ro*a/(1-a)
Figure
22.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
K’d
and
n
for
UHP
treated
(600
MPa)
BLG
with
16
min
holding
time
at
pH
5.0.
70
70 60 50 40
y
=
1.4841x
-
5.1371
R2
=
0.983
30
Po*a
20 10 0 -10
0
5
10
15
20
25
30
35
40
45
50
Ro*a/(1-a)
Figure
23.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
K’d
and
n
for
UHP
treated
(600
MPa)
BLG
with
32
min
holding
time
at
pH
5.0.
71
120
100
80
y
=
1.0454x
-
6.4156
R2
=
0.9716
60
Po*a
40
20
0
0
10
20
30
40
50
60
70
80
90
100
-20
Ro*a/(1-a)
Figure
24.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
K’d
and
n
for
native
BLG
at
pH
7.0.
72
100
80
60
y
=
1.1917x
-
7.7794
R2
=
0.9306
40
Po*a
20
0
0
10
20
30
40
50
60
70
80
-20
Ro*a/(1-a)
Figure
25.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
K’d
and
n
for
UHP
treated
(600
MPa)
BLG
with
0
min
holding
time
at
pH
7.0.
73
90 80 70 60 50
y
=
1.2602x
-
5.7985
R2
=
0.9858
40
Po*a
30 20 10 0 -10
0
10
20
30
40
50
60
70
80
Ro*a/(1-a)
Figure
26.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
K’d
and
n
for
UHP
treated
(600
MPa)
BLG
with
4
min
holding
time
at
pH
7.0.
74
35 30 25 20
y
=
0.9591x
-
4.2045
R2
=
0.9217
15 10
Po*a
5 0 -5
0
5
10
15
20
25
30
35
40
-10
Ro*a/(1-a)
Figure
27.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
K’d
and
n
for
UHP
treated
(600
MPa)
BLG
with
8
min
holding
time
at
pH
7.0.
75