Document Text (Pages 81-90) Back to Document

Application of ultra high hydrostatic pressure for investigating the binding of flavor compounds to ß-lactoglobulin via headspace solid phase microextraction-gas chromatography

by 1977- Hoang, Tinyee Arden, PhD


Page 81

120

100

80
y
=
0.997x
-
5.5514

R2
=
0.9967
60
Po*a
40

20

0

0
20
40
60
80
100
120
-20
Ro*a/(1-a)

Figure
18.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
native
BLG
at
pH
5.0.

66


Page 82

80 70
y
=
1.4244x
-
5.4289

R2
=
0.994
60 50 40
Po*a
30 20
67

10 0

0
10
20
30
40
50
60
-10
Ro*a/(1-a)

Figure
19.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
untreated
BLG
and
UHP-treated
(600
MPa)
BLG
with
0
min
holding
time
at
pH
5.0.


Page 83

80 70 60
y
=
1.4151x
-
7.1145

R2
=
0.954

50 40 30
Po*a
20 10 0 -10

0
5
10
15
20
25
30
35
40
45
50
Ro*a/(1-a)

Figure
20.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
untreated
BLG
and
UHP-treated
(600
MPa)
BLG
with
4
min
holding
time
at
pH
5.0.

68


Page 84

70 60 50 40
y
=
1.4706x
-
5.3086

R2
=
0.9816

30
Po*a
20 10 0 -10

0
5
10
15
20
25
30
35
40
45
50

Ro*a/(1-a)

Figure
21.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP
treated
(600
MPa)
BLG
with
8
min
holding
time
at
pH
5.0.

69


Page 85

80 70 60
y
=
1.6185x
-
11.583

R2
=
0.9996

50 40
Po*a
30 20 10 0

0
10
20
30
40
50
60
Ro*a/(1-a)

Figure
22.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP
treated
(600
MPa)
BLG
with
16
min
holding
time
at
pH
5.0.

70


Page 86

70 60 50 40
y
=
1.4841x
-
5.1371

R2
=
0.983

30
Po*a
20 10 0 -10

0
5
10
15
20
25
30
35
40
45
50

Ro*a/(1-a)

Figure
23.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP
treated
(600
MPa)
BLG
with
32
min
holding
time
at
pH
5.0.

71


Page 87

120

100

80
y
=
1.0454x
-
6.4156

R2
=
0.9716

60
Po*a
40

20

0

0
10
20
30
40
50
60
70
80
90
100
-20
Ro*a/(1-a)

Figure
24.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
native
BLG
at
pH
7.0.

72


Page 88

100

80

60
y
=
1.1917x
-
7.7794

R2
=
0.9306

40
Po*a
20

0

0
10
20
30
40
50
60
70
80
-20
Ro*a/(1-a)

Figure
25.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP
treated
(600
MPa)
BLG
with
0
min
holding
time
at
pH
7.0.

73


Page 89

90 80 70 60 50

y
=
1.2602x
-
5.7985
R2
=
0.9858

40
Po*a
30 20 10 0 -10

0
10
20
30
40
50
60
70
80
Ro*a/(1-a)

Figure
26.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP
treated
(600
MPa)
BLG
with
4
min
holding
time
at
pH
7.0.

74


Page 90

35 30 25 20
y
=
0.9591x
-
4.2045

R2
=
0.9217

15 10
Po*a
5 0 -5

0
5
10
15
20
25
30
35
40

-10
Ro*a/(1-a)

Figure
27.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP
treated
(600
MPa)
BLG
with
8
min
holding
time
at
pH
7.0.

75

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