Document Text (Pages 71-80) Back to Document

Application of ultra high hydrostatic pressure for investigating the binding of flavor compounds to ß-lactoglobulin via headspace solid phase microextraction-gas chromatography

by 1977- Hoang, Tinyee Arden, PhD


Page 71

56

Figure
8.
Extrinsic
PRODAN
emission
spectra
of
BLG
solutions
at
pH
5.0
treated
with
UHP
at
600
MPa
for
selected
holding
times
from
0
to
32
min
(UHP0-UHP32).


Page 72

57

Figure
9.
Extrinsic
PRODAN
emission
spectra
of
BLG
solutions
at
pH
7.0
treated
with
UHP
at
600
MPa
for
selected
holding
times
from
0
to
32
min
(UHP0-UHP32).


Page 73

58

Figure
10.
Extrinsic
PRODAN
emission
spectra
of
BLG
solutions
at
pH
9.0
treated
with
UHP
at
600
MPa
for
selected
holding
times
from
0
to
32
min
(UHP0-UHP32).


Page 74
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1.8
2
BLG
UHP0
UHP4
UHP8
UHP16
UHP32
Surface
Hydrophobicity
pH3 pH5 pH7 pH9
Figure
11.
Surface
hydrophobicity
of
BLG
determined
at
pH
3.0-9.0
after
ultra
high
pressure
(UHP)
treatment
times
0,
4,
8,
16
or
32
min
with
PRODAN.
59

Page 75

180 160 140 120 100 80

y
=
0.4416x
-
50.69
R2
=
0.9329

Po*a
60 40 20 0

0
50
100
150
200
250
300
350
400
450
500
Ro*a/(1-a)

Figure
12.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
native
BLG
at
pH
3.0.

60


Page 76

160 140 120 100

y
=
0.5205x
-
37.876
R2
=
0.8298

80 60
Po*a
40 20 0 -20

0
50
100
150
200
250
300
350
Ro*a/(1-a)

Figure
13.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP
treated
(600
MPa)
BLG
with
0
min
holding
time
at
pH
3.0.

61


Page 77

160 140 120

y
=
0.5455x
-
29.945
R2
=
0.9255

100 80 60
Po*a
40 20 0 -20

0
50
100
150
200
250
300
Ro*a/(1-a)

Figure
14.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP
treated
(600
MPa)
BLG
with
4
min
holding
time
at
pH
3.0.

62


Page 78

160 140 120 100
y
=
0.7572x
-
83.523

R2
=
0.9063

80 60
Po*a
40 20 0 -20

0
50
100
150
200
250
300
350
Ro*a/(1-a)

Figure
15.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP-treated
(600
MPa)
BLG
with
8min
holding
time
at
pH
3.0.

63


Page 79

160 140 120 100

y
=
0.5196x
-
35.653
R2
=
0.9446

80 60
Po*a
40 20 0 -20

0
50
100
150
200
250
300
350
400
Ro*a/(1-a)

Figure
16.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP-treated
(600
MPa)
BLG
with
16
min
holding
time
at
pH
3.0.

64


Page 80

160 140 120 100
y
=
0.8632x
-
120.35

R2
=
0.9392
80
Po*a
60 40 20 0

0
50
100
150
200
250
300
350
Ro*a/(1-a)

Figure
17.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP-treated
(600
MPa)
BLG
with
32
min
holding
time
at
pH
3.0.

65

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