Document Text (Pages 91-100) Back to Document

Application of ultra high hydrostatic pressure for investigating the binding of flavor compounds to ß-lactoglobulin via headspace solid phase microextraction-gas chromatography

by 1977- Hoang, Tinyee Arden, PhD


Page 91

100

80
y
=
1.1078x
-
5.3498

R2
=
0.9786
60

40
Po*a
20

0

0
10
20
30
40
50
60
70
80
90
-20
Ro*a/(1-a)

Figure
28.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP
treated
(600
MPa)
BLG
with
16
min
holding
time
at
pH
7.0.

76


Page 92

90 80 70 60 50

y
=
1.2546x
-
10.558
R2
=
0.9173

40 30
Po*a
20 10 0 -10

0
10
20
30
40
50
60
70
80
-20
Ro*a/(1-a)

Figure
29.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP-treated
(600
MPa)
BLG
with
32
min
holding
time
at
pH
7.0.

77


Page 93

90 80 70 60
y
=
1.2555x
-
4.6596

R2
=
0.972
50 40
Po*a
30 20 10 0 -10

0
10
20
30
40
50
60
70
Ro*a/(1-a)

Figure
30.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
native
BLG
at
pH
9.0.

78


Page 94

90 80 70 60 50
y
=
1.237x
-
6.1243

R2
=
0.9692

40
Po*a
30 20 10 0 -10

0
10
20
30
40
50
60
70
Ro*a/(1-a)

Figure
31.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP
treated
(600
MPa)
BLG
with
0
min
holding
time
at
pH
9.0.

79


Page 95

80 70 60 50
y
=
1.3736x
-
5.7587

R2
=
0.985

40
Po*a
30 20 10 0 -10

0
10
20
30
40
50
60
Ro*a/(1-a)

Figure
32.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP
treated
(600
MPa)
BLG
with
4
min
holding
time
at
pH
9.0.

80


Page 96

70 60 50
y
=
1.561x
-
5.3656

R2
=
0.9636
40 30
Po*a
20 10 0 -10

0
5
10
15
20
25
30
35
40

Ro*a/(1-a)
Figure
33.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP-treated
(600
MPa)
BLG
with
8
min
holding
time
at
pH
9.0.

81


Page 97

80
y
=
1.3807x
-
9.2447

R2
=
0.8976
70 60 50 40 30
Po*a
20 10
82

0

0
10
20
30
40
50
60
-10 -20
Ro*a/(1-a)

Figure
34.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP
treated
(600
MPa)
BLG
with
16
min
holding
time
at
pH
9.0.


Page 98

90 80 70 60
y
=
1.2175x
-
5.5852

R2
=
0.978
50 40
Po*a
30 20
83

10 0

0
10
20
30
40
50
60
70
80
-10
Ro*a/(1-a)

Figure
35.
PRODAN
binding
to
BLG
plotted
by
Cogan
method
(1976)
to
calculate
Kd
and
n
for
UHP-treated
(600
MPa)
BLG
with
32
min
holding
time
at
pH
9.0.


Page 99
Table
1.
Apparent
dissociation
constants
(Kd
)
and
the
number
of
binding
sites
(n)
of
BLG
for
PRODAN
after
UHP
treatment
(600
MPa)
for
selected
holding
times
of
0
to
32
min
(UHP0-UHP32)
at
pH
3.0-9.0
calculated
using
the
method
by
Cogan
and
others
(1976)*.
pH3
pH5
pH7
pH9
K
' d
(10 -7
M) 1
n
2
K
' d
(10 -7
M) 1
n
2
K
' d
(10 -7
M)
1
n
2
K
' d
(10 -7
M)
1
n
2
BLG
112.14
2.25
5.55
1.04
6.15
0.95
3.71
0.89
UHP0
72.53
1.86
3.55
0.78
6.75
0.86
4.63
0.79
UHP4
53.99
1.80
5.09
0.74
4.51
1.05
4.10
0.75
UHP8
110.42
1.25
3.84
0.72
4.86
1.04
3.31
0.62
UHP16
67.79
1.90
7.14
0.62
4.84
0.95
6.70
0.73
UHP32
138.87
1.16
3.41
0.69
8.44
0.78
4.57
0.84
*:
Data
are
means
of
three
analyses
calculated
using
method
by
Cogan
and
others
(1976).
1 ,
2 :
Means
with
different
letters
in
the
column
are
significantly
different
(p
<
0.05).
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
pH3
pH5
pH7
pH9
K
' d
(10 -7
M) 1
n
2
K
' d
(10 -7
M) 1
n
2
K
' d
(10 -7
M)
1
n
2
K
' d
(10 -7
M)
1
n
2
BLG
112.14
2.25
5.55
1.04
6.15
0.95
3.71
0.89
UHP0
72.53
1.86
3.55
0.78
6.75
0.86
4.63
0.79
UHP4
53.99
1.80
5.09
0.74
4.51
1.05
4.10
0.75
UHP8
110.42
1.25
3.84
0.72
4.86
1.04
3.31
0.62
UHP16
67.79
1.90
7.14
0.62
4.84
0.95
6.70
0.73
UHP32
138.87
1.16
3.41
0.69
8.44
0.78
4.57
0.84
*:
Data
are
means
of
three
analyses
calculated
using
method
by
Cogan
and
others
(1976).
1 ,
2 :
Means
with
different
letters
in
the
column
are
significantly
different
(p
<
0.05).
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
a b c d e f
84

Page 100

CHAPTER THREE

Application of solid-phase microextraction with headspace gas chromatography for
the analysis of diacetyl, 2-methylbutyraldehyde, ethyl lactate, and δ-decalactone in
Model Buffer Solution

1 2 2 1

Tinyee HoangP P, D. Scott MattinsonP P, John K. FellmanP P, Joseph R. PowersP P, and Barry

1

SwansonP

P

(1) Dept. of Food Science & Human Nutrition, (2) Dept. of Horticulture & Landscape
Architecture, Washington State Univ., PO Box 646376, Pullman, WA 99164-6376

85

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