All-trans retinoic acid downregulates CCAAT/enhancer binding proteins in human bronchial epithelial cells
Abstract (Summary)The goal of our studies was to elucidate mechanisms that control and modulate normal bronchial epithelial cell (BECs) proliferation and differentiation in respiratory epithelium. We have evaluated the effects of All-trans retinoic acid (RA) treatment of BECs in culture. BECs cultured in serum free media were exposed to 30 nM RA, and gene expression analysis was performed with a standardized quantitative reverse transcription polymerase chain reaction (StaRT-PCR) after 24 hours exposure. Treatment of BECs with RA enhances differentiation. This enhancement was correlated with a decrease in the transcript abundance levels of squamous differentiation marker, small, proline-rich 1 gene (Spr1) and the leucine zipper family transcription factors CCAAT/enhancer binding proteins, C/EBPá, C/EBPâ and C/EBPã, and an increase in the levels of retinoic acid modulated gene tissue transglutaminase 2 (TGM2), anti-apoptotic protein B-cell leukemia/lymphoma 2 (Bcl-2) and differentiation genes, forkhead box A1 (foxA1) and cell surface associated gene (MUC1). Our results indicate that RA induced BEC differentiation independent of the cell cycle arrest and apoptosis pathway.
School Location:USA - Ohio
Source Type:Master's Thesis
Keywords:cebpa rar start pcr
Date of Publication:01/01/2007