Aberrant methylation of E-cadherin gene (ECAD) in invasive ductal breast carcinoma
(Uncorrected OCR) Abstract of thesis entitled Aberrant methylation of E-cadherin gene (ECAD) in invasive ductal breast (not otherwise specified) (IDC-NOS) carcinoma submitted by Lui Lik Hang Eric For the degree of Master of Medical Sciences at the University of Hong Kong in August 2005 Invasive ductal carcinoma is poorly differentiated and extensively invasive. Fibroadenomas are the most common benign tumors of the female breast, accounting for 90% of breast masses in women. Neoplastic cells display a variety of genomic alterations, among which those involving DNA methylation constitute the most spectacular epigenetic change. Such aberrant methylation and silencing of tumor suppressor genes (TSGs), including genes that regulate critical processes such as cell cycle control, DNA repair, and angiogenesis. E-cadherin gene (ECAD) is a TSG, it encodes the protein E-cadherin play important roles in the architecture and function of the normal mammary gland. The aim of this study is to investigate the hypermethylation status of E-cadherin gene (ECAD) in invasive ductal carcinomas (IDC) and fibroadenoma (FA). Breast tumour tissues were collected from 50 EDC patients. DNA extraction and methylation-specific PCR (MSP) for ECAD were performed preceded by pathological examination. The clinicopathological data were compared with the hypermethylation status. 19 breast FA samples were also being processed in the similar way. The percentage of hypermethylated ECAD is 32% (16/50) in IDC cases and 0.00% (0/12) in FA cases. In hypermethylated ECAD cases, 88% (14/16) showed HER-2/neu overexpression with positive correlation (r=0.7, p<0.01). This study provides an understanding of the relation between ECAD hypermethylation and tumor characteristics. Furthermore, it was confirmed that hypermethylation of ECAD does not occur in FA. If this hypermethylation is detected at early stages, it may potentially be a reference to the planning of corresponding treatment.
School:The University of Hong Kong
School Location:China - Hong Kong SAR
Source Type:Master's Thesis
Keywords:dna methylation antioncogenes breast cancer genetics aspects
Date of Publication:01/01/2005